Loop mediated DNA amplification(LAMP) 

      Loop-mediated isothermal amplification (LAMP) assay is a novel method of gene amplification that amplifies nucleic acid, which can be applied for disease diagnosis in shrimp aquaculture. During the LAMP reaction, the white precipitate of magnesium pyrophosphate (Mg2P2O7), which indicates the virus in shrimp, is formed correlates with the amount of synthesized DNA. So, the turbidity can be measured by Real-time Turbidimeter. In this work, a Real-time Turbidimeter is developed to detect Shrimp Virus, which caused large economic losses to most major shrimp-producing countries including Thailand. The principle of Real-time Turbidimeter
     The device incorporate a heating block that maintain the optimal temperature of 63 ºC for the duration of the 25 shrimp sample perform in a 0.2 ml tube. We also applied the spectroscopic measurement technique to measure a by-product of LAMP reaction. The white turbidity of shrimp sample can be real-time monitored when light from an inexpensive light-emitting diode (LED) pass through the tube to reach a photo diode.

 Features of Real-time Turbidimeter
 Can be amplified and real-time monitored every 1 second simultaneously in 8 samples.
 Can be monitored the end point detection and display on LCD.
 The real-time measurement can be displayed on personal computer via USB port, which is  easy 
 installation and simple operation.With the small size, suitable for field application.

 Real-time monitoring software
 Shows real-time monitoring as line graph.
 Data exporting as CSV files can be analyzed in Microsoft Office Excel.

 Main Specification

     The relatively short analysis time, the low cost, no need for a thermocycler, no need for detection reagents and no need to open the reaction tube “ all are key advantages of this LAMP-turbidity measurement system. Therefore, this method could be used for both screening and confirmatory diagnosis of shrimp virus. Thus, it was recommended for application in shrimp health management programs for disease surveillance in hatcheries and grow-out ponds. If the agriculturists in shrimp local farm have the apparatus which is of the low cost, rapid and timely disease diagnosis, population of the shrimps in the farms will increase and virus will not spread out to other environment.